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  • DEPC
  • 產品型號:101-1609-47-8

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for RNA experiment, RNase deactivator. Synonym: DEP, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride

O(COOC2H5)2
M.W.: 162.14
Assay: >99% Min.
Dimethylcarbonate: 1.0% Max.
Methanol: 0.2% Max.
Arsenic (As): 0.0002% Max.
Chloride (Cl): 0.001% Max.
Heavy Metal: 0.0005% Max.
Residue on Ignition: 0.1% Max.

Modification reagent for His and Tyr residues in proteins. In 0.1% solution, it inactivates RNase, thus protecting RNA from degradation.

For the treatment of water with DEPC, add 1 ml of DEPC to 1 liter bi-distilled water (0.1 % DEPC v/v) and stir over night. Autoclaving 20 minutes inactivates DEPC. DEPC reacts with water and hydrolyses to ethanol and CO2.
Store at 4ºC

Caution: DEPC is suspected to be a carcinogen and should be handled with great care. Do not breathe fumes and avoid contact with skin! DEPC may damage eyes and mucous membranes!

Harmful, Irritant!