Components ( In 56.4g M9 Salts Base(5X) /1L H2O)：
Na2HPO4: 33.9g
KH2PO4: 15g
NaCl: 2.5g
NH4Cl: 5g

  M9 minimal Salts Base 5X is a 5x concentrate that is diluted to a 1x concentration and supplemented with an appropriate carbon and energy source, such as dextrose, to provide a minimal, chemically defined medium. The medium will support the growth of “wild-type” strains of E. coli. M9 Minimal Salts is useful for maintaining positive selection pressure on plasmids coding for the ability to produce essential substances such as amino acids or vitamins.
  Sodium phosphate(Na2HPO4) and potassium phosphate(KH2PO4) are present as buffering agents. Ammonium chloride(NH4Cl) is a source of nitrogen for cellular systems. Sodium chloride(NaCl) provides essential ions. Glucose may be added as a source of carbohydrate. Supplementing the medium with magnesium and calcium increases the growth of recombinants.

Application
M9, Minimal Salts, 5X has been used:

    in a selective agar medium for the isolation of sulphate reducing bacteria
    in GMML minimal media to propagate phage-resistant DH10B cells
    in M9 minimal medium which is used for cell motility assay

M9 Minimal Salts 5x are suitable for the preparation of M9 Minimal Medium for use in propagation of E. coli and plasmid amplification. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for maintaining positive selection pressure on E. coli that produce essential amino acids and for maintenance of F′-containing bacteria strains for M13 propagation.

Store at Room Temp.