C6H16N2
M.W.: 116.20
Assay: 99.9% min.
pH: 8.0    

    TEMED was introduced as an enhancer of the polymersitation (cross-linking) of acrylamide and bisacrylamide in gel electrophoresis. It catalysis the formation of free radicals of the initiator of the polymerisation, ammonium persulfate. If gels are degased to remove oxygen, add the TEMED after degasing.

Working conc.: 50uL/100mL

Needles, H.L. (1970) Anal. Biochem. 35, 533-537 Effect of solution components on large-pore polyacrylamide gel formation. Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87 Electrophoresis in agarose and acrylamide gels.
Gomes, A.V. & Barnes, J.A. (1998) Anal. Biochem. 260, 106-108 Gel electrophoresis of mini gels.