Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to another thermostable polymerase. Its molecular weight is 90 kDa；PCR products are blunt-ended without 3’end dA；Pfu DNA Polymerase also possesses 3´→5´ exonuclease proofreading activity (high fidelity) that enables the polymerase to correct nucleotide-misincorporation errors, but no 5’→3’ exonuclease activity.

Unlike Taq DNA polymerase ex: AmpliTaq or KlenTaq, the specific is determined by itself. Some other DNA Polymerase can be used with Pfu, and it can promote the amplification efficiency and correct nucleotide mis-incorporation error but use Pfu only will achieve higher fidelity.

Concentration: 5U/μL, 100 μL

Store at -20℃.