產品櫥窗
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G418
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產品型號：101-108321-42-2 商品規格： |
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Selection Antibiotics, Cell Culture Synonym: disulfate salt, Antibiotic G418, G418 disulfate salt, neomycin
C20H40N4O10·2H2SO4
M.W.: 692.71
Potency: >700ug/mg
TLC: Max. Three Spot
Geneticine (G418) is used for the selection of stably transformed cells, which have incorporated neomycin resistance gene bearing the transposons Tn5 and Tn601, respectively. Since this antibiotic is toxic for many cells, one has to determine the optimal concentration for each cell type. In general this concentration varies from 50 to 5000 ug/mL. At a concentration of 500 ug/mL, 70% of HepG2-cells are killed within one week of incubation and 100% stop growing. Geneticin is stable at 4℃.
Note: All researchers should determine their optimum working concentration experimentally for each lot!
Stock concentration: 50mg/mL in water
Working concentration:
5ug/mL (bacteria and algae)
400ug/mL for selection(mammalian cells)
200ug/mL for maintenance(mammalian cells)
M.W.: 692.71
Potency: >700ug/mg
TLC: Max. Three Spot
Geneticine (G418) is used for the selection of stably transformed cells, which have incorporated neomycin resistance gene bearing the transposons Tn5 and Tn601, respectively. Since this antibiotic is toxic for many cells, one has to determine the optimal concentration for each cell type. In general this concentration varies from 50 to 5000 ug/mL. At a concentration of 500 ug/mL, 70% of HepG2-cells are killed within one week of incubation and 100% stop growing. Geneticin is stable at 4℃.
Note: All researchers should determine their optimum working concentration experimentally for each lot!
Stock concentration: 50mg/mL in water
Working concentration:
5ug/mL (bacteria and algae)
400ug/mL for selection(mammalian cells)
200ug/mL for maintenance(mammalian cells)
Store at -20℃
Toxic for reproduction
Toxic for reproduction