產品櫥窗
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DTT (DL-Dithiothreitol)
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產品型號：101-3483-12-3 商品規格： |
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Protein Experiment, Protein Disulfide Bond, Reducing Reagent. Synonym: threo-1,4-Dimercapto-2,3-butanediol, DL-Dithiothreitol, Cleland’s reagent.
C4H10O2S2
M.W.: 154.25
99% Min
Melting Point: 41-42ºC
For Molecular Biology Research Use
Soln. (5% in water): Clear and Colorless
Dithiothreitol (DTT) is, like beta-mercaptoethanol, a reducing reagent for proteins and protects the cysteine residues against oxidation.
DTT is an excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Stock Conc.: 1M in ddH2O, Store at -20ºC
Working Conc.: 0.1-1mM
Cleland, W.W. (1964) Biochemistry 3, 480-482
Dithiothreitol, a new protective reagent for SH groups.
M.W.: 154.25
99% Min
Melting Point: 41-42ºC
For Molecular Biology Research Use
Soln. (5% in water): Clear and Colorless
Dithiothreitol (DTT) is, like beta-mercaptoethanol, a reducing reagent for proteins and protects the cysteine residues against oxidation.
DTT is an excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Stock Conc.: 1M in ddH2O, Store at -20ºC
Working Conc.: 0.1-1mM
Cleland, W.W. (1964) Biochemistry 3, 480-482
Dithiothreitol, a new protective reagent for SH groups.
Store at 4ºC
Shipping at Room Temp within Taiwan; may vary elsewhere
Shipping at Room Temp within Taiwan; may vary elsewhere