RIPA(Radioimmunoprecipitation Assay) lysis buffer  is used to lyse cells and tissues for use in  radioimmunoprecipitation, protein assays, protein purification and other analytical procedures. Due to its ionic detergent composition, RIPA can disrupt nuclear membranes and solubilize cytoplasmic proteins, resulting in a high protein yield. 

Composition:
0.5M Tris-HCl, 1.5M Sodium chloride, 10% NP-40, 5% Sodium deoxycholate, 1% SDS, 50mM EDTA

Note: RIPA Lysis Buffer does not contain any protease inhibitors or phosphatase inhibitors.  If desired, add inhibitors to RIPA Lysis Buffer immediately before applying to cells.

*Aggregation may be present in this buffer upon arrival due to the high concentrations of reagents included at the supplied 10X formulation. At times, the aggregation persists despite warming to room temperature. We recommend warming the buffer to 37°C for 15 minutes and mixing to help eliminate the precipitate. Alternatively, diluting the 10X RIPA with ddH2O to at least 5X will yield a precipitate-free solution. Once diluted, it can be aliquoted and stored at -20°C for future use.