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  • EGTA
  • 產品型號:101-67-42-5

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Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

Formular: [-CH2OCH2CH2N(CH2CO2H)2]2
MW: 380.35

EGTA, or Ethylene glycol bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid, is a chelating agent. It's commonly used in biochemistry and cell biology to selectively bind calcium ions and control their concentration in solutions, particularly in experiments involving cells where calcium plays a crucial role. Compared to EDTA, EGTA has a much higher affinity for calcium ions and a lower affinity for magnesium ions, making it a useful tool for studying calcium-dependent processes.

Applications:
Controlling Calcium Concentrations: EGTA is frequently used to buffer calcium concentrations in solutions, creating controlled environments for experiments on cells or biochemical reactions. 

Studying Calcium Signaling: By manipulating calcium levels with EGTA, researchers can investigate how calcium signals contribute to various cellular functions.

Protein Purification: EGTA is used in protein purification techniques, particularly in affinity chromatography, to elute (remove) calcium-binding proteins from a column. 


 
Store at Room Temp. Keep cool and dry.