Complete Supplement Mixture (CSM, DO Supplement), Double Drop-out -x -y

These high-quality dropout supplements can be used together with SD base to create selective media for growing yeast cultures, confirming auxotrophic phenotypes, and selecting yeast transformants. They are ideal for use with Matchmaker Gold Systems and other yeast one-hybrid systems or yeast two-hybrid systems.

Dropout (DO) supplements provide various mixtures of amino acids and nucleotides to add to the Minimal SD Bases to make a defined minimal medium. In each DO mix, certain amino acids have been “dropped out”, for example the -Leu/-Trp DO supplement contains every essential amino acid except for Leucine and Tryptophan. DO Supplements may also be added to the minimal E. coli medium M9 for minimal selection in E. coli.

mg/L
Adenine 10
L-Arginine 50
L-Aspartic acid 80
L-Histidine HCl 20
L-Isoleucine 50
L-Leucine 100
L-Lysine HCl 50
L-Methionine 20
L-Phenylalanine 50
L-Threonine 100
L-Tryptophan 50
L-Tyrosine 50
Uracil 20
Valine 140

TOTAL 790

Store at Room Temp.
Keep dry.