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5X SDS-PAGE Loading Dye
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產品型號：20078 商品規格： |
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For Protein Electrophoresis (SDS-PAGE), For Sample Loading. synonym：5X SDS-PAGE Loading dye
5X SDS-PAGE Loading Buffer is ideal for preparing protein samples to be separated in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and allows for easy sample monitoring during electrophoresis.
SDS-PAGE Loading Buffer also protects proteins from heat degradation during the sample preparation step, as well against pH changes during the SDS-PAGE run. Some proteins are sensitive to pH changes that result from temperature fluctuations during electrophoresis in Tris buffers. The optimized composition of the loading buffer prevents protein degradation during sample heating prior to SDS-PAGE as well as during the electrophoresis run.
Components:
312 mM Tris-HCl (pH6.8)
10% (W/V) SDS
45% (V/V) Glycerol
1% (W/V) Bromophenol Blue
5% (V/V) beta-mercaptoethanol
SDS-PAGE Loading Buffer also protects proteins from heat degradation during the sample preparation step, as well against pH changes during the SDS-PAGE run. Some proteins are sensitive to pH changes that result from temperature fluctuations during electrophoresis in Tris buffers. The optimized composition of the loading buffer prevents protein degradation during sample heating prior to SDS-PAGE as well as during the electrophoresis run.
Components:
312 mM Tris-HCl (pH6.8)
10% (W/V) SDS
45% (V/V) Glycerol
1% (W/V) Bromophenol Blue
5% (V/V) beta-mercaptoethanol