This broad spectrum CyICT® Protease + Phosphatase Inhibitor Cocktail TURBO has been specifically designed to provide complete protection of valuable proteins of interest from degradation during extraction and purification. It inhibits activity in extracts from almost any tissue or cell types including animals, plants, yeasts, bacteria and fungi.

Application:
Western Blot, Co-IP pull-down, IF, IHC, kinase assay...

Features and Benefits:
Complete protection from proteases and phosphatases in one single cocktail.

100x Solution. The cocktail contains 8 inhibitors targeting against the major classes of proteases and phosphatases as outlined in the following table.

Products	Target Proteases	Products	Target Phosphatases
Aprotinin	Broad Spectrum, Serine Proteases	Sodium Orthovanadate	Protein Tyrosine Phosphatase, Alkaline Phosphatase
Bestatin	Aminopeptidase B and Leucine Aminopeptidase	Sodium Fluoride	Acid Phosphatases
E-64	Cysteine Proteases	Sodium Pyrophosphate	PP1 and PP2A
Leupeptin	Cysteine Proteases and Trypsin-like Proteases	Glycerophosphate	Ser/Thr Phosphatases

Recommended Usage:
This cocktail has been optimized for effectiveness, convenience, stability, reproducibility and has been tested for general use. It inhibits activity in extracts from almost any tissue or cell types including animals, plants, yeasts, bacteria and fungi. Dilute this  stock solution to 1x before use. Recommended dilution is 1:100 (based on the application).

 

Store at -20°C. Hygroscopic.