TBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Tris Buffered Saline with Tween 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.

Procedure:
TBST Buffer 10X contains 1.50M Sodium Chloride, 0.20M Tris/Tris-HCl and 1% (w/v) Tween 20
Dilute with ddH2O. 

10X TBST stock solution (1 Liter) contains:
87.7 g of NaCl
24.2 g of Tris base
10mL of Tween 20
Adjust pH to 7.5 by HCl.
​Ready to use. Before use, add 9X of ddH2O to dilute. 

Store at Room Temp.