產品櫥窗
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Bis-Tris Propane
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產品型號：101-64431-96-5 商品規格： |
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Molecular Biology Buffer. Synonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
CH2[CH2NHC(CH2OH)3]2
M.W.: 282.33
Assay (Dried Basis Titration): 99.0% Min.
Moisture: 1.0% Max.
A280nm (1% in H2O): 0.15 Max.
A400nm (1% in H2O): 0.05 Max.
pH (0.5% in H2O): 10.4 - 11.0
Bis-Tris propane is a zwitterionic buffer that is used in biochemistry and molecular biology. It has an unusually wide buffering range, from approximately pH 6 to 9.5, because its two pKa values are close in value.
A review of DNA polymerase fidelity and its role in the chain reaction (PCR) discusses the use of Bis-Tris propane as a suitable buffer. The use of a Bis-Tris propane/HCl buffer for the stabilization of farnesyl diphosphate isolated from a mutant strain of Saccharomyces cerevisiae has been described. Bis-Tris propane buffer has been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodopsin. An investigation of the MgATPase activity of the myosin subfragment 1 monomer in Bis-Tris propane buffer has been reported. The effect of buffer identity on the kinetics of the restriction enzyme EcoR V has been studied in various buffers, including Bis-Tris propane.
Useful pH Range: 6.3-9.5
M.W.: 282.33
Assay (Dried Basis Titration): 99.0% Min.
Moisture: 1.0% Max.
A280nm (1% in H2O): 0.15 Max.
A400nm (1% in H2O): 0.05 Max.
pH (0.5% in H2O): 10.4 - 11.0
Bis-Tris propane is a zwitterionic buffer that is used in biochemistry and molecular biology. It has an unusually wide buffering range, from approximately pH 6 to 9.5, because its two pKa values are close in value.
A review of DNA polymerase fidelity and its role in the chain reaction (PCR) discusses the use of Bis-Tris propane as a suitable buffer. The use of a Bis-Tris propane/HCl buffer for the stabilization of farnesyl diphosphate isolated from a mutant strain of Saccharomyces cerevisiae has been described. Bis-Tris propane buffer has been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodopsin. An investigation of the MgATPase activity of the myosin subfragment 1 monomer in Bis-Tris propane buffer has been reported. The effect of buffer identity on the kinetics of the restriction enzyme EcoR V has been studied in various buffers, including Bis-Tris propane.
Useful pH Range: 6.3-9.5
Store at Room Temp.
Solutions of this product are expected to be stable to autoclaving. Solutions stored at 2-8 °C are stable for months.
Solutions of this product are expected to be stable to autoclaving. Solutions stored at 2-8 °C are stable for months.