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  • RIPA Lysis Buffer 10X
  • 產品型號:RIPA10

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Lysis Buffer for Western Blot sample preparation

    RIPA(Radioimmunoprecipitation Assay) lysis buffer  is used to lyse cells and tissues for use in  radioimmunoprecipitation, protein assays, protein purification and other analytical procedures. Due to its ionic detergent composition, RIPA can disrupt nuclear membranes and solubilize cytoplasmic proteins, resulting in a high protein yield. 

Composition:
0.5M Tris-HCl, 1.5M Sodium chloride, 10% NP-40, 5% Sodium deoxycholate, 1% SDS, 50mM EDTA

Note: RIPA Lysis Buffer does not contain any protease inhibitors or phosphatase inhibitors.  If desired, add inhibitors to RIPA Lysis Buffer immediately before applying to cells.
Store at 4°C upon receipt.