產品櫥窗
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M9 minimal Salts Base 5X
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產品型號：3019 商品規格： |
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M9 Minimal Salts, 5× is used in preparing M9 Minimal Medium which is used for cultivating recombinant strains of Escherichia coli.
M9 Minimal Salts, 5× is a 5× concentrate that is diluted to a 1× concentration and supplemented with an appropriate carbon and energy source, such as dextrose, to provide a minimal, chemically defined medium. The medium will support the growth of “wild-type” strains of E. coli. M9 Minimal Salts is useful for maintaining positive selection pressure on plasmids coding for the ability to produce essential substances
such as amino acids or vitamins.
Sodium phosphate(Na2HPO4) and potassium phosphate(KH2PO4) are present as buffering agents. Ammonium chloride(NH4Cl) is a source of nitrogen for cellular systems. Sodium chloride(NaCl) provides essential ions. Glucose may be added as a source of carbohydrate. Supplementing the medium with magnesium and calcium increases the growth of recombinants.
Components ( In 56.4g M9 Salts Base(5X) /1L H2O)：
Na2HPO4: 33.9g
KH2PO4: 15g
NaCl: 2.5g
NH4Cl: 5g
such as amino acids or vitamins.
Sodium phosphate(Na2HPO4) and potassium phosphate(KH2PO4) are present as buffering agents. Ammonium chloride(NH4Cl) is a source of nitrogen for cellular systems. Sodium chloride(NaCl) provides essential ions. Glucose may be added as a source of carbohydrate. Supplementing the medium with magnesium and calcium increases the growth of recombinants.
Components ( In 56.4g M9 Salts Base(5X) /1L H2O)：
Na2HPO4: 33.9g
KH2PO4: 15g
NaCl: 2.5g
NH4Cl: 5g
Store at Room Temp.