產品櫥窗
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SOC Broth
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產品型號：3016 商品規格： |
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SOC is a microbial growth medium used for the transformation of competent cells (E. coli).
SOC Broth (Super Optimal broth with Catabolite repression) is SOB(Super Optimal broth) with added glucose.
Super Optimal Broth (SOB medium) is a nutrient-rich bacterial growth medium used for microbiological culture, generally of Escherichia coli. It was developed by Douglas Hanahan in 1983 and is an adjusted version of the commonly used LB medium (lysogeny broth). Growth of E. coli in SOB or SOC medium results in higher transformation efficiencies of plasmids.
For maximum effectiveness, SOB/SOC media should have its pH adjusted to 7.0 by adding concentrated sodium hydroxide. The original literature states that the pH of the final medium should be between 6.8 and 7.0.
Hanahan, D. (1983). "Studies on transformation of Escherichia coli with plasmids". Journal of Molecular Biology. 166 (4): 557–580.
Components(In 31.54g SOC Broth/1L H2O):
Yeast Extract: 5g
Tryptone: 20g
NaCl: 0.5g
MgSO4: 2.44g
Glucose: 3.6g(20mM)
Store at 4oC. Keep dry.
Shipping at Room Temp.
Shipping at Room Temp.