Ni NTA His-Tag Purification Resin 6FF can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli, yeast, insect cells, and mammalian cells. Ni NTA His-Tag Purification Resin 6FF consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni NTA His-Tag Purification Resin 6FF is highly stable and expand the range of suitable operating conditions.

Store the Ni NTA His-Tag Purification Resin 6FF for longer periods of time in an equal volume of 1X PBS containing 20% ethanol at 2-8°C.