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  • E-Fect DNA Transfection Reagent
  • 產品型號:LE-10203

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Transfection reagent for DNA delivery to cells for gene overexpression.

    E-Fect is a cationic polymer gene transfection reagent. It has several unique features necessary for efficient transfection, such as DNA condensation and endosomal release, which can improve gene transfection efficiency. Compared with liposome-based method, cationic polymers are very stable, easy to handle and more resistant to serum in cell culture. The above advantages make gene transfection much easier and reproducible. E-Fect can be successfully used in both established cell lines and primary cells. Some cell lines have been successfully transfected with E-Fect as shown in Table 1. Transfection can be finished in half an hour. E-Fect is a serum resistant reagent; the transfection procedure is much simpler than traditional methods: E-Fect / DNA complex can be directly added into complete cell medium. With no need to change and wash cell medium, the 2-step hands-on transfection procedure can be completed in less than 30 minutes.

Cell lines successfully transfected with E-Fect:
 Cell line  Description
 A549  Human lung cancer cell line
 C6/36  Aedes albopictus; Mosquito cell line
 DLBCL  Diffuse large B cell lymphoma
 ES-2  Human ovarian cancer cells
 HCT116  Human colon cancer cells
 HeLa  Human cervical cancer cell line
 HT-29  Human cancer colon cell line
 HUVEC  Human umbilical vein endothelial cells
(Primary cells)
 K562  Human erythroleukemic (blood) cell line
 PBMC  Human peripheral blood mononuclear cells
 S2  Drosophila melanogaster cell line
 SF9  Spodoptera frugiperda (insect) cell line
 SP2/0  Mouse myeloma cell line
 THP-1  Human leukemic monocytic cell line
Cell line Efficiency (% GFP)
 293T 90-98
 BHK-21 80-95
 Huh7 75-95
 HepG2 75-90
 Vero 40-80
 HMEC-1 40-65
 U118 MG 25-40
 SKNSH 25-60
 CHO-K1 70-90
 MDCK 65-80

Store at -20°C.