C47H73NO17
M.W.: 924.08
USP

Freez dryed,  non-sterile
Additive: Sodiumdeoxyclolate(41mg) and phospate buffers

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Amphotericin B is isolated from Streptomyces nodosus and an anti-fungal agent used for prevention or elimination of fungal contaminants in cell cultures. It's also used as an inhibitor of yeasts, molds and mycoplasma in cell cultures. It belongs to the macrocyclic lactones and its action is fungistatic. Amphotericin B binds to sterols (e. g. cholesterol, cholestanol) with planar structure and disturbs the membrane permeability. Ions, like K+, Na+ and H+ or other low molecular weight substances (e. g. amino acids, sugars, nucleotides) may cross the membrane.

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility.

Stock Conc.: 30-40mg/mL in DMSO
Working Conc.: 0.25-2.5ug/mL

Store at 4℃. Harmful !