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  • X-Gal (X-beta-Gal)
  • 產品型號:101-7240-90-6

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Enzymatic Colorimetric Reagent, For GAL Detection. Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG

C14H15BrClNO6
M.W.: 408.63
Assay: 98.5% min.
Stock conc.: 2% (w/v) in dimethylformamide or DMSO.
 
    X-Gal is a chromogenic substrate of b-Galactosidase. It is used in conjunction with IPTG for detection of b-Galactosidase activity in bacterial colonies in a colorimetric assay to detect recombinants (white) from non-recombinants (blue). X-Gal is cleaved at the b 1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl part of X-Gal by b-Glactosidase via hydrolysis.
    The cleavage of X-Gal results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate at the site of enzymatic cleavage.
    In cloning strategies with vectors such as Lamda-11, M13mp18 and 19 and 19, pUC18 and 19, pUR222 the E.Coli lacZ gene is transformed to lac cells. After transformation the cells show b-Galactosidase activity in the presence of IPTG and X-Gal containing media. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of b-Galactosidase activity leading to the appearance of white colonies on X-Gal and IPTG containing media. Non recombinant cells produce a blue indigo dye on these media.
 
 
Store at -20℃. Protect from light.
Shipping at Room Temp within Taiwan; may vary elsewhere