C₂₂H₂₄N₂O₈·HCl
M.W.: 480.90
Assay: 97.2%
Moisture: <2.0%
pH (1% Soln. in water): 1.8-2.8
4-Enianhydrotetracycline HCl: 0.5% min.

 

Tetracycline is a bacteriostatic antibiotic with activity against gram-positive and gram-negative bacteria. Within the cell tetracycline binds reversible to the 30S subunit of the ribosome, preventing the binding of aminoacyl transfer RNA and inhibiting protein synthesis and hence cell growth. Used as a selective marker for the transformation of plasmids encoding for tetracycline resistance (Tetr) such as pBR322, pBR325 and pMB9. 

 

Stock conc.: 10 mg/mL in H2O. Stock solutions should be filtered sterilized and stored at −20°C.
Working conc.: 10 μg/mL

 

Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89, 5547-5551
Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Gossen, M. et al. (1995) Science 268, 1766-1769
Transcriptional activation by Tetracyclines in mammalian cells.

 

Store at -20℃.
Protected from light!