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  • Taq DNA Polymerase
  • 產品型號:101-9012-90-2

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​For amplification of DNA fragments by PCR, label the DNA fragment with radioactive isotope, biotin etc.

Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus, a strain lacking Taq I restriction endonuclease. Taq catalyzes the polymerization of nucleotides into duplex DNA in the 5’à3’ direction in the presence of magnesium. The enzyme has an apparent molecular weight of 94,000 daltons by SDS-PAGE and exhibits 5'->3' exonuclease activity, lacks 3'->5' exonuclease activity. Taq is recommended for use in PCR and primer extension reactions at elevated temperature.

Taq DNA Polymerase has the independent terminal transferal activity which results in the addition of a single nucleotide (adenosine) at 3' end of the extension product. TA cloning vector is recommended if the extension product is needed to be cloned.

Unit: 5U/uL, 100uL/vial
Sources: Thermus aquaticus
Error Rate: 1/10^5


 
 Store at -20℃.