Nitrocellulose and PVDF membranes that have been probed by Western blotting procedures and detected by chemiluminescent or other nonprecipitating substrates may be used to detect tubulin, actin and other stable-expression proteins as a consult or to compare with the other proteins. 

Western Blot Stripping Buffer provides a robust but gentle method for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane. Compared with a new SDS-PAGE, it is much simpler and easier and can avoid the error causing by new loading. 

Advantages: 
Stripping high-affinity antibody;
Effective use of samples that are available in limited amounts;
Multiple times of re-probes (~20 times) ;
Comparison of images obtained with different antibodies in the same blot ;
Confirmation of results with the same or different antibodies ;
It is simply more economical and less time consuming to reuse blots for re-probing and mass spectrometry.


 

Store at room temp.
Store at 4℃ for long term storage