產品櫥窗
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Excel Super-Fidelity DNA Polymerase
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產品型號：VZ-505P 商品規格： |
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High fidelity DNA polymerase, which can amplify long fragment up to 40kb. It is compatible with high GC-content samples and can directly be used in crude extract.
ExcelTM Super-Fidelity DNA Polymerase is a new generation superior enzyme based on ExcelTM DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to ExcelTM Super-Fidelity greatly improve its long-fragment amplification ability, specificity, and PCR yield. P ExcelTM Super-Fidelity is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of ExcelTM Super-Fidelity is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, ExcelTM Super-Fidelity has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. ExcelTM Super-Fidelity contains two monoclonal antibodies inhibiting the 5’->3’ polymerase activity and 3’->5’ exonuclease activity at room temperature, which enable ExcelTM Super-Fidelity to perform greatly-specific Hot-Start PCRs. The amplification generates blunt-ended products, which need additional A-tailing step for compatible with general TA cloning kit.
Features:
High speed amplification
Amplification of long fragment up to 40kb
Compatible with high GC-content
Can directly be used in crude extract
Components:
| 100 U | 500 U | 1000 U | |
| Excel Super-Fidelity DNA Polymerase (1 U/µl) | 100 µl | 5× 100 µl | 10× 100 µl |
| 2× Excel Super-Fidelity Buffer | 1.25 ml | 5× 1.25 ml | 10× 1.25 ml |
| dNTP Mix (10 mM each) | 100 µl | 5× 100 µl | 10× 100 µl |
| 10× Loading buffer | 1.25 µl | 5× 1.25 µl | 10× 1.25 µl |
